Concentrate was mixed with 0. After elution in OSB, protein containing fractions as determined by western blotting were pooled and protein concentrations were determined using absorbance at nm and calculated molar extinction coefficients of both proteins. Myc-tagged EGa1 control nanobodies were produced and purified as described previously. Protein—lipid overlay assay Protein—lipid overlay assay was performed as described elsewhere, 45 with minor modifications. Phospholipids Protein—lipid binding was visualized using an Odyssey Infrared Imager at nm. Plates were washed three times with[…]
